Human Fc fragment of IgM receptor (FAIM3) ELISA Kit

Cat.#:

JL17147

Regulatory Status:

RUO

Alternative:

TOSO; FCMR; Regulator of Fas-induced apoptosis Toso;Fas Apoptotic Inhibitory Molecule 3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.072ng/mL

Specificity:

This kit recognizes Human FAIM3 in samples. No significant cross-reactivity or interference between Human FAIM3 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| FAIM3

Fc fragment of IgM receptor is a protein that is encoded by the FCMR gene.Fc receptors specifically bind to the Fc region of immunoglobulins (Igs) to mediate the unique functions of each Ig class. FAIM3 encodes an Fc receptor for IgM.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.35	1.55	1.03	0.64	0.45	0.33	0.24	0.09
Corrected OD	2.26	1.46	0.94	0.55	0.36	0.24	0.15	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human FAIM3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	97
Cell Culture Supernates(n=8)	92-106	102

| Linearity

Samples were spiked with high concentrations of Human FAIM3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	86-96
1 : 2	Average (%)	90	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98